Streamline your influenza testing with leading diagnostics

Influenza Testing

 

Diagnose infections quickly and accurately for better patient care with powerful manual and automated technologies, and a comprehensive line of media and clinical diagnostic products. Backed by more than 150 years of combined technical and scientific expertise, Thermo Fisher Scientific is the partner of choice for today’s leading clinical laboratories.

What method is right for you?

Selection Criteria

  Rapid Lateral-Flow Rapid Molecular qPCR NGS
Time 3 min 45 min 2 hours 24 hours
Sensitivity -

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Specificity

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Ease of use

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Detection

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Characterisation - -

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Rapid Lateral-Flow

Results in as little as 3 minutes*

Rapid Visual Tests or Lateral Flow Assays are used to rapidly diagnosis a wide variety of medical conditions. Several breakthroughs have occurred over the past few years that have enabled the implementation of advanced fluorescent based immunoassay system at the point of care.

The principle of Lateral Flow Assay utilises a sandwich design to detect a specific analyte in the test specimen. Which means the analyte is bound between two components, i.e. antibody – antigen – antibody; or antigen – antibody – antibody (bold indicates target analyte). Each of Quidel’s Lateral Flow Assays contains an on board quality control to indicate that the test has run properly.

 

Rapid Lateral Flow Assays

 

 

The advantages of fluorescent detection systems include:

  • Higher sensitivity detection of the analyte
  • Simplified reagents
  • Simpler assay designs

 

When a fluorescent detection system is linked to a lateral flow assay and matched with a powerful yet low cost analyser like the Quidel Sofia® or Sofia 2®, the result is improved assay performance, the opportunity for walk away testing along with the elimination of misinterpretation often associated with visually-read point-of-care assays.

Learn more about Sofia Fluorescent Immunoassay Testing Platform

Sofia 2 Video

Watch Sofia 2 Demo Video

 

*Time to result is dependent on pathogen being tested and recommended workflow. Refer to product insert for time line for specific kits.

Rapid Molecular

With results available from as little as 30 min*

 

Helicase Dependent Amplification Assays

Helicase Dependent Amplification (HDA) is an isothermal DNA amplification method that is similar to conventional PCR, but utilises the action of a thermostable helicase enzyme, rather than heat to separate the nucleic acids and enable labelled primers to anneal to the DNA template and elongate under the action of the polymerase.

 

This allows DNA replication to occur at a single uniform temperature (64°C) with a simple heat block, rather than a series of varying time and temperature conditions utilised by expensive real-time thermocyclers.

 

The process supplies accurate molecular test results that you can see, achieved without the need for specialised equipment or complex software for data interpretation.

 

Solana is a validated bench top instrument that combines Quidel’s proprietary helicase-dependent amplification (HDA) with fluorescence detection to deliver molecular results you can trust, faster than ever before.

Learn more about Solana Molecular Testing Platform

 

HDA Video

Watch Quidel’s Proprietary
Helicase-Dependent Amplification Video

*Time to result is dependent on pathogen being tested and recommended workflow. Refer to product insert for time line for specific kits.

Traditional Molecular (qPCR)

With results available from as little as 2 hours*

Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). In real-time PCR (qPCR), reactions are characterised by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. The higher the starting copy number of the nucleic acid target, the sooner a significant increase in fluorescence is observed.

 

Real-time systems for PCR were improved by the introduction of fluorogenic-labeled probes that use the 5´ nuclease activity of Taq DNA polymerase. The availability of these fluorogenic probes enabled the development of a real-time method for detecting only specific amplification products. The development of fluorogenic labeled probes also made it possible to eliminate post-PCR processing for the analysis of probe degradation.

How probe based qPCR works

How TaqMan Works?

Learn how TaqMan works from Sr. Field Application Specialist, Doug Rains.

 

These fluorogenic labels have been utilised for typing of influenza infections for diagnostics and epidemiological reporting across human and zoonotic strains.

Human   Animal

 

 
QuantStudio Dx

QuantStudio™ Dx Real-Time Instrument

QuantStudio™ Dx Real-Time PCR Instrument the diagnostic real-time PCR system from Applied Biosystems delivers proven performance, security and reliability to meet the needs of your clinical laboratory today and tomorrow.

Learn more

 

 

*Time to result is dependent on pathogen being tested and recommended workflow. Refer to product insert for time line for specific kits.

Next Generation Sequencing (NGS)

With results available from as little as 24 hours*

Fastest targeted sequencing workflow for viral genomes and gene panels

Ion AmpliSeq™ technology provides a simple enrichment method for targeted sequencing of archived biological materials that have not been cultured. It is highly effective in identifying variants without the time and cost associated with more comprehensive approaches such as whole genome sequencing or multilocus sequence typing (MLST), which require pure samples.

 

Ion AmpliSeq™ technology is an easy-to-use enrichment method for targeted sequencing of archived biological samples that have not been cultured. Based on ultrahigh-multiplex PCR, Ion AmpliSeq technology requires as little as 1 ng of input nucleic acid to target sets of genes, making sequencing of limited-quantity or degraded samples more accessible on Ion Torrent™ sequencing systems. You can get data from your precious samples in less than 24 hours.

 

The combined power of Ion AmpliSeq technology and Ion Torrent sequencing provides a scalable targeted sequencing system to sequence pathogens.

Ion Torrent NGS

Learn more

 

 

PCR amplification of influenza A genomic segments for whole-genome sequencing

 

The influenza A genome is ~13.5 kb and comprises 8 segments that encode up to 11 proteins. The terminal segment-specific nucleotide regions are unique to each segment and are conserved across influenza A viruses, making it possible for researchers to design primers for reverse transcription and PCR amplification (Figure 1) and then to sequence the entire viral genome.

 

Viral genome sequencing allows strain typing and traceability facilitating epidemiological recording and effective vaccine selection.

 

Figure 1.
Primer design strategy for sequencing the influenza A genome.

 

(A) Influenza A genome with 8 RNA fragments.
(B) Primer design overview for the amplification of all
      8 influenza A genomic segments.


Learn more

 

Figure 1

Ion Torrent Influenza A Primer

 

 

*Time to result is dependent on pathogen being tested and recommended workflow. Refer to product insert for time line for specific kits.

 

 

 

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